User:Dileep D. Monie/Notebook/2008-4-1: Difference between revisions
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*Added 5ml of Supplemented M9 Media containing 20 μg/ml Kanamycin (Sigma K0254; lot no. 027K2378) to fifteen 17mm test tubes | *Added 5ml of Supplemented M9 Media containing 20 μg/ml Kanamycin (Sigma K0254; lot no. 027K2378) to fifteen 17mm test tubes | ||
*Added 5ml of Supplemented M9 Media with no antibiotic (for the TOP10 negative control cells) to three 17mm test tubes | *Added 5ml of Supplemented M9 Media with no antibiotic (for the TOP10 negative control cells) to three 17mm test tubes | ||
*Inoculated media by picking single colonies from plates streaked on | *Inoculated media by picking single colonies from plates streaked on 2008-3-24 (each strain was inoculated in triplicate from different colonies) | ||
*Grew the cultures at 37°C with shaking at 70 RPM | *Grew the cultures at 37°C with shaking at 70 RPM | ||
Latest revision as of 13:46, 3 April 2008
- Added 5ml of Supplemented M9 Media containing 20 μg/ml Kanamycin (Sigma K0254; lot no. 027K2378) to fifteen 17mm test tubes
- Added 5ml of Supplemented M9 Media with no antibiotic (for the TOP10 negative control cells) to three 17mm test tubes
- Inoculated media by picking single colonies from plates streaked on 2008-3-24 (each strain was inoculated in triplicate from different colonies)
- Grew the cultures at 37°C with shaking at 70 RPM
