User:Dileep D. Monie/Notebook/2008-4-3: Difference between revisions

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m (New page: *Analyzed flow cytometry data collected on 2008-4-2 using BD CellQuest Pro v5.2 *Forward vs side scatter plot showing TOP10 cells (1% of events) and R1 gate used for statistics [[Image:DDM...)
 
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*Analyzed flow cytometry data collected on 2008-4-2 using BD CellQuest Pro v5.2
*Analyzed flow cytometry data collected on 2008-4-2 using BD CellQuest Pro v5.2
*Forward vs side scatter plot showing TOP10 cells (1% of events) and R1 gate used for statistics
*Forward vs side scatter plot showing TOP10 cells (1% of events shown) and R1 gate used for statistics
[[Image:DDM_TOP10-A.JPG]]
[[Image:DDM_TOP10-A.JPG]]
*Histogram overlay showing FL1 signal (GFP) of representative samples from each group (gated on R1) and M1/M2 markers used for statistics
*Histogram overlay showing FL1 signals (GFP) of representative samples from each group (gated on R1) and markers (M1 and M2) used for statistics
[[Image:DDM_overlay.JPG]]
[[Image:DDM_overlay.JPG]]
#Purple solid: TOP10
#Purple solid: TOP10
Line 10: Line 10:
#Orange line: I20268
#Orange line: I20268
#Dark blue line: I20260
#Dark blue line: I20260
*Calculated geometric means of fluorescence intensities (I<sub>sample</sub>)for triplicate samples (bounded by appropriate marker)
<pre>
TOP10 (M1) I20259 (M1) I20269 (M2) I20270 (M2) I20268 (M2) I20260 (M2)
1.62 1.66 34.62 78.93 133.87 170.4
1.65 1.68 33.44 77.06 133.29 168.55
1.65 1.67 34.15 77.18 137.02 171.03
</pre>
*Calculated fluorescence intensities in consensus BioBrick™ GFP units (I/I<sub>I20260</sub>)
<pre>
TOP10 I20259 I20269 I20270 I20268 I20260
0 0.000178197 0.192630579 0.451926504 0.790519938 1
</pre>

Revision as of 14:35, 3 April 2008

  • Analyzed flow cytometry data collected on 2008-4-2 using BD CellQuest Pro v5.2
  • Forward vs side scatter plot showing TOP10 cells (1% of events shown) and R1 gate used for statistics

  • Histogram overlay showing FL1 signals (GFP) of representative samples from each group (gated on R1) and markers (M1 and M2) used for statistics

  1. Purple solid: TOP10
  2. Green line: I20259
  3. Pink line: I20269
  4. Dotted line: I20270
  5. Orange line: I20268
  6. Dark blue line: I20260
  • Calculated geometric means of fluorescence intensities (Isample)for triplicate samples (bounded by appropriate marker)
TOP10 (M1)	I20259 (M1)	I20269 (M2)	I20270 (M2)	I20268 (M2)	I20260 (M2)
1.62		1.66		34.62		78.93		133.87		170.4
1.65		1.68		33.44		77.06		133.29		168.55
1.65		1.67		34.15		77.18		137.02		171.03
  • Calculated fluorescence intensities in consensus BioBrick™ GFP units (I/II20260)
TOP10	I20259		I20269		I20270		I20268		I20260
0	0.000178197	0.192630579	0.451926504	0.790519938	1
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