User:Dileep D. Monie/Notebook/BBF Standards Measurement/2008/04/03: Difference between revisions

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==Entry title==
==Entry title==
*Analyzed flow cytometry data collected on 2008-4-2 using BD CellQuest Pro v5.2
*Analyzed flow cytometry data collected on 2008-04-02 using BD CellQuest Pro v5.2
*Forward vs side scatter plot showing TOP10 cells (1% of events are shown) and R1 gate used for statistics
*Forward vs side scatter plot showing TOP10 cells (1% of events are shown) and R1 gate used for statistics
[[Image:DDM_TOP10-A.JPG]]
[[Image:DDM_TOP10-A.JPG]]
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#Green line: I20259
#Green line: I20259
#Pink line: I20269
#Pink line: I20269
#Dotted line: I20270
#Light blue line: I20270
#Orange line: I20268
#Orange line: I20268
#Dark blue line: I20260
#Dark blue line: I20260

Revision as of 11:08, 8 April 2008

BBF Standards: Promoter Measurement Test <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Entry title

  • Analyzed flow cytometry data collected on 2008-04-02 using BD CellQuest Pro v5.2
  • Forward vs side scatter plot showing TOP10 cells (1% of events are shown) and R1 gate used for statistics

  • Histogram overlay showing FL1 signals (GFP) of representative samples from each group (gated on R1) and markers (M1 and M2) used for statistics

  1. Purple solid: TOP10
  2. Green line: I20259
  3. Pink line: I20269
  4. Light blue line: I20270
  5. Orange line: I20268
  6. Dark blue line: I20260
  • Calculated geometric means of fluorescence intensities (Isample,MX) for triplicate samples (bounded by appropriate marker)
TOP10 (M1)	I20259 (M1)	I20269 (M2)	I20270 (M2)	I20268 (M2)	I20260 (M2)
1.62		1.66		34.62		78.93		133.87		170.4
1.65		1.68		33.44		77.06		133.29		168.55
1.65		1.67		34.15		77.18		137.02		171.03
  • Calculated fluorescence intensities in consensus BioBrick™ GFP units (I/II20260) using (<Isample,MX>-<ITOP10,M1>)/(<II20260,M2>-<ITOP10,M1>)
TOP10	I20259		I20269		I20270		I20268		I20260
0	0.000178197	0.192630579	0.451926504	0.790519938	1


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