User:Dominique Brager/Notebook/481 Notebook/2017/02/15

Biomaterials Design Lab

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Objective Make 40% Beads Filter All Beads for drying Vacuum Filtrate and grind up 25% and 40% yellow beads Sieve dried yellow beads Description 40% Beads: Used procedure from first day 2.5g PVOH, 54 mL bentonite solution, 26mL water, 30mL HCl Do two rounds of 150mL ethyl acetate (half of bead solution in one) For each round put in 1.5mL glutaraldehyde twice (6mL total) Yellow Beads Vacuum Filtrated 25% and 40% beads. 25% Beads: 1.2262g 40% Beads: 1.8708g Ground beads using mortal and pestle Left to dry overnight Vacuum Filtrated New Beads Placed on watch-glass to dry Data Calculated Swelling using masses right after freeze drying and 'wet' masses after letting them sit in ethyl acetate/water solution for a week and then vacuum filtrating. Swelling % = (wet-dry)/dry 10%: 60.9% 25%: 21.8% 40%: 66.6% Notes Cabbage Dye is natural and therefore susceptible to mold and to going bad Cabbage left in drawer and not refrigerated went bad and changed colors. Will have to make new dye and keep it in fridge this time. Any experiments using dye will have to be done earlier rather than later to avoid dye going bad.