User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/08/01: Difference between revisions

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#*2.53 mM stock soln
#*2.53 mM stock soln
#Volumes Needed For Colloid & Fiber Solutions
#Volumes Needed For Colloid & Fiber Solutions
{| class="wikitable"
{| {{table}}
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! !! Lys Colloid !! Lys Fiber !! BSA Colloid !! BSA fiber
! !! Lys Colloid !! Lys Fiber !! BSA Colloid !! BSA fiber

Revision as of 22:09, 13 October 2014

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AuNP Synthesis

  1. HAuCl4 Stock Solution
    • 42.97 mg HAuCl4 using parafilmed spatula
    • 50 mL water in vol flask
    • 2.53 mM stock soln
  2. Volumes Needed For Colloid & Fiber Solutions
Lys Colloid Lys Fiber BSA Colloid BSA fiber
Au:protein < 35 > 35 < 135 > 135
[protein stock] 70.96 μM 17.73 μM
Vol HCl 988 μL 988 μL 988 μL 988 μL
Vol protein 1.174 mL 0.881 mL 1.083 mL 1.066 mL
Au:protein 30 40 130 140
  1. AuNP Preparations
    • 30:1 Au-Lys --> 988 μL Au + 2*587 μL protein + 7.838 mL H2O
    • 40:1 Au-Lys --> 988 μL Au + 2*440 μL protein + 8.131 mL H2O
    • 140:1 Au-BSA --> 988 μL Au + 2*503 μL protein + 8.006 mL H2O
    • Heated in foil covered glass test tubes 80 °C for 3 hr
    • Added 24.32 mg CaCl2 ("anhydrous" label, but visibly wet) to 30:1 Au-Lys solution
    • Fibers in other two, as expected
    • Invertase not dissolved, so not used



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