User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/11/04: Difference between revisions

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**Prepare 10 mL 0.01 wt% Micrococcus lysodeikticus Cell Suspension (in freezer) '''using phosphate buffer'''
**Prepare 10 mL 0.01 wt% Micrococcus lysodeikticus Cell Suspension (in freezer) '''using phosphate buffer'''
**Adjust the cell suspension concentration until the Absorbance at λ = 450 nm is between 0.6 & 0.7
**Adjust the cell suspension concentration until the Absorbance at λ = 450 nm is between 0.6 & 0.7
**The full protocol is [[http://www.sigmaaldrich.com/technical-documents/protocols/biology/enzymatic-assay-of-lysozyme.html| here]]
**The full protocol is [http://www.sigmaaldrich.com/technical-documents/protocols/biology/enzymatic-assay-of-lysozyme.html| here]<br>




Revision as of 09:20, 4 November 2014

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Tasklist

  • Measure dialysis concentrations from last week
  • Prepare a new dialysis using colloid solution and CaCl2 or HCl
    • For those running CaCl2, use 3500 MWCO & test concentrations between 10 and 50 mM
    • For those running HCl, use 25000 MWCO & test concentrations between 100 μM and 10 mM
  • Prepare solutions for enzyme kinetics
    • Prepare 25 mL 66 mM Potassium phosphate, pH 6.24 (beaker prep is fine)
    • Place half in a Falcon tube, label & refrigerate overnight
    • Prepare 10 mL 0.01 wt% Micrococcus lysodeikticus Cell Suspension (in freezer) using phosphate buffer
    • Adjust the cell suspension concentration until the Absorbance at λ = 450 nm is between 0.6 & 0.7
    • The full protocol is here



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