User:Dr. Pascal Dowling

Dr. Pascal Dowling, BScPharm(Hons), MPhil, PhD , Pharmaceutical Industry , Switzerland

Contact Info

Email me through OpenWetWare

Profile

A global medical director working at a biotech company. Previous experience has been gained in medical affairs within the pharmaceutical industry and biotech, with additional medical/scientific communications and medical marketing commercial experience within the medical OTC consumer industry. I describe myself as having a broad scientific knowledge of products and associated target groups deriving from my pharmacology education, including pre-clinical and clinical (CRO experience) fields, currently within neurology, and previously within rare diseases,neurology and dermatology, complemented by an existing global KOL network. Furthermore, I possess analytical and practical approaches to problem solving, a strong attention to detail and process planning, a business acumen, and both local operating and international/global team work experiences. People management in my current and previous positions has been gained. One area of passion is improving public health in all regions, whilst educating and raising awareness amongst HCPs about important neurological conditions.

Scientific Education & Training

• 2010, Post-doctorate research scientist, Pharmacology, Max-Planck Institute for Heart and Lung Research, Bad Nauheim, Germany

• Research Topics: Cardiopulmonary, epithelial, Orphan GPCRs, neuropharmacology, angiogenesis • Clinical Relevance: Cardiovascular disease, COPD, novel brain receptor targets • Supervisor: Professor Dr. Offermanns • Department: Pharmacology • Techniques: BAC-Transgenics, southern blotting, Flexstation-3, standard molecular biology, IHC, ICC, 293 HEK cells, • Grants: Max Planck Society

• 2009, Ph.D., Neuroscience, Georg-August-University, Göttingen, Germany

• Research topics: Neuroscience, Clinical Neuroscience, Neuropharmacology, Dopamine, Alzheimer’s, Parkinson’s, mitochondrial, movement disorders, iron-deficiency, D3R-/- mice • Clinical Relevance: Restless legs syndrome, Parkinson’s disease, nutritional deprivation • Supervisor: Professor Dr. Paulus, Professor Dr. Liebetanz • Ph.D. Course structure: Purely research based Doctorate, where a 150 page classical thesis was produced and examined along with publications by a 1-2 hour viva and an intellectual Rigour (Rigorosum) on the topics, intracellular signaling mechanisms and central nervous regulation of sleep and pain. • Program: Georg-August University School of Science (GAUSS) PhD program • Department: Clinical Neurophysiology • Start/End: August 2006/March 2009 • Thesis title: “Impact of iron-deficiency upon behaviour and protein expression in the male mouse with implications for Restless Legs Syndrome”. • Techniques: Animal behavioural experiments (grip-test, running-wheel, hot-plate, formalin-test) were implemented and an dietary induced animal model was established. Retro-orbital sinus blood extraction, transcardial perfusion, tissue extraction, preparation and analysis. Immunohistochemistry, both peroxidase and fluorescence techniques. Molecular techniques were performed; these were 1-D/2-D DIGE electrophoresis, MALDI-TOF-MS and Western Blotting both TANK and Semi-Dry. • Collaborators: Max Planck Institute for Experimental Medicine: Dr. Jahn (Proteomics Group) and Dr. Werner (Department of Neurogenetics) Centre for Clinical Research: Professor Dr. Otto (Neurology Group) University Medical Centre: Professor Dr. Stadelmann-Nessler (Department of Neuropathology) • Examiners: Professor Dr. Flügge, Professor Dr. Kessel, Professor Hardeland • Grants: Deutsche Forschungsgemeinschaft (DFG) funded European Graduate College (GK 632) and GSK • Teaching: Involved in mentoring and lecturing medical and PhD students • Published: Yes (Please refer to the attached publication list)

• 2009, Postgraduate Researcher, Neuropharmacology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany

• Department: The Prof. Peter Gruss (MPI President) Department of Molecular Cell Biology • Research topics: Neuroscience, Neuropharmacology, Parkinson’s disease, human ES cells, transplantation, dopamine, animal-model • Clinical Relevance: Parkinson’s disease • Supervisor: Professor Dr. Mansouri • Techniques: Murine and human spermatogenic stem cell cultures, differentiation, electroporation. ICC, FACS, cloning pGEM T-Easy vector, Southern blotting. Post-implant IHC dopamine neuron and receptor staining. • Collaborators: Professor Dr. Engel, Professor Dr. Paulus • Grants: Deutsche Forschungsgemeinschaft (DFG) and Max-Planck Society

• 2006, M.Phil., Neuroscience, Cardiff University, Cardiff, UK

• Research topics: Neuroscience, autonomic nervous system, peripheral ganglia, animal model, senescence • Clinical Relevance: Urinary incontinence • Supervisor: Dr. Santer • M.Phil. Course structure: Purely research based Masters, where a 150 page classical thesis was produced and examined along with other relevant material (publications) by a 3-4 hour viva. • Program: Cardiff Biosciences • Department: Neuroscience Group • Start/End: December 2004/October 2005 • Thesis title: “Age Related Changes in Preganglionic and Postganglionic Neurons in the Major Pelvic Ganglion of the Male Rat.” • Techniques: Transmission electron microscopy (TEM), general IHC procedures and quantitative fluorescence microscopy. • Published: Yes (Please refer to the attached publication list)

• 2004, B.Sc.(Hons), Pharmacology, University of Manchester, Manchester, UK

• Course Topics: Drug Discovery, Drug Disposition, Neuropharmacology, Advanced Neuropharmacology, Bioinformatics, Endocrinology Biostatistics, Brain Structure and function, Drug Design, Ion Channels and Enzymes as Drug Targets, Pharmaceutical Chemistry, and Molecular Toxicology. • Techniques: DH5α bacteria and 293 HEK cells culturing, ELISA, gene cloning, rodent animal behavioural studies, site-directed mutagenesis, transformation of DH5α bacteria, transfection of mutant DNA, patch clamp and radioligand binding assays, high throughput screening and development of novel compounds from GSK and AstraZeneca. • Final year research project titled: “Interaction of epibatidine with the resting state of the foetal muscle nicotinic acetylcholine receptor.” Employed radioligand binding assays and electrophysiological (patch clamp) studies to show the importance of epibatidine selectivity upon certain residues on γ and δ subunits of the muscle nAChRs when in the resting conformation state. • Final year dissertation: Usage of GABAergic compounds as anti-convulsant therapies • Supervisors: Professor Dive, Professor Weston • Grants: Medical Research Council (MRC)

• 2001, A-Levels: Chemistry, Mathematics, Physics (Truro, Cornwall, UK)

Industry Interests

1. Global medical affairs
2. Global pharmaceutical markets
3. Drug discovery & development in Pharma/Biotech
4. Medical marketing
5. Medical communications
6. Strategic medical business development
7. KOL interaction and management
8. Perceptual mapping
9. Medical Education - MSL training
10. HEOR
11. Real World Evidence
12. Emerging markets
13. Data-analysis
14. CRM and HCP platform designs
15. Project design and management
16. Line management
17. Medical information
18. Market access
19. Pain
20. Neurodegenerative diseases
21. Cardiology & COPD
22. Dermatology
23. Rare diseases

Research Interests

1. Neuro- and psycho-pharmacology
2. Neurodegenerative diseases
3. Ageing of PNS and CNS, Alzheimer's, Parkinson's, RLS, schizophrenia, memory, pain
4. Orphan GPCRs
5. Pre-clinical model design for industry
6. Stem cells
7. Addiction

Research Courses & Techniques

• Centre for Clinical Research: 2-D DIGE and 2-D IEF/SDS-PAGE • MPI Heart & Lung: BAC-transgenics, Flexstation-3 • MPI Experimental Medicine: Proteomics MALDI-TOF-MS and genomics • MPI Biophysical Chemistry: Patch-Clamp - Lab of Nobel laureate Professor Erwin Neher • MPI Biophysical Chemistry: Murine and human ES cell culturing, FACS, Southern blotting, transplantation • UMG: Animal behavioural studies, surgical, blood analysis, ELISA, blotting, confocal microscopy • Cardiff University: Transmission Electron Microscopy • Cardiff University: IHC, dissection, microscopy • University of Manchester: Radioligand binding, basic cell culture and molecular techniques • University of Manchester: Personnel Home Office License for Working under the Animals Scientific Procedure Act 1986 1999. Modules 1-5 (including legal and ethical). • AstraZeneca: High throughput screening and development of novel compounds • SPSS, MINITAB and Prism statistics training courses. • MATLAB programming training

Affiliations

• Royal Pharmaceutical Society of Great Britain
• British Pharmacological Society
• Project Management Institute
• British Neuroscience Association
• German Neuroscience Society
• Max-Planck Society

• University of Manchester
• Cardiff University
• Georg-August University Göttingen
• Max-Planck Institute for Biophysical Chemistry
• Max-Planck Institute for Heart & Lung Research

Publications

• Klinker F, Hasan K, Dowling P, Paulus W, Liebetanz D. Dopamine D(3) receptor deficiency sensitizes mice to iron deficiency-related deficits in motor learning. Behav Brain Res. 2011 Jul 7;220(2):358-61.

• Dowling P, Klinker F, Stadelmann C, Hasan K, Paulus W, Liebetanz D. Dopamine D3 receptor specifically modulates motor and sensory symptoms in iron-deficient mice. J Neurosci. 2011 Jan 5;31(1):70-7.

• Dowling P, Klinker F, Amaya F, Paulus W, Liebetanz D. Iron-deficiency sensitizes mice to acute pain stimuli and formalin-induced nociception. J Nutr. 2009 Nov;139(11):2087-92.

• Paulus W, Dowling P, Rijsman R, Stiasny-Kolster K, Trenkwalder C. Update of the pathophysiology of the restless-legs-syndrome. Mov Disord. 2007;22 Suppl 18:S431-9.

• Paulus W, Dowling P, Rijsman R, Stiasny-Kolster K, Trenkwalder C, de Weerd A. Pathophysiological concepts of restless legs syndrome. Mov Disord. 2007 Jul 30;22(10):1451-6. Review.

• Ranson RN, Dowling P, Santer RM, Watson AH. The effects of ageing on the distribution of vesicular acetylcholine transporter immunoreactive inputs to pelvic motoneurons of male Wistar rats. Neuroscience. 2007 Jan 19;144(2):636-44. Epub 2006 Oct 30.

• Dowling P, Ranson RN, Santer RM. Age-associated changes in distribution of the P2X2 receptor in the major pelvic ganglion of the male rat. Neurosci Lett. 2006 Sep 1;404(3):320-3.