User:Dspelke: Difference between revisions
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| Line 30: | Line 30: | ||
|- | |- | ||
| I | | I | ||
| | | Assembly | ||
| | | Modifiy the gene so that the channels becomes less selective and thus allow other ions, molecules, and proteins to enter and exit the bacteria- see how this affects the life cycles of both the bacteria and the phage | ||
|- | |- | ||
| II | | II | ||
| | | Replication of DNA + strand | ||
| | | Alter the gene to make it more active and thus replicate DNA more frequently- see how increased DNA production affects phage growth | ||
|- | |- | ||
| III | | III | ||
| | | Phage tail protein (5 copies) | ||
| | | Modify the proteins that bind to the bacteria (and thus initiate the F pilus and infection) so that the bacteriophage canbind to and infect other types of bacteria- examine the varied life cycles that result | ||
|- | |- | ||
| IV | | IV | ||
| | | Assembly | ||
| | | Alter the gene in such a way as to destabalize the outer membrane (e.g. no longer detergent-resistant)- test varying environments for phage survival rate | ||
|- | |- | ||
| V | | V | ||
| | | Binds ssDNA | ||
| Vary the activity of the gene and thus the competition between dsDNA formation and the sequestering of ssDNA- compare the results to find the optimum level of phage production possible | | Vary the activity of the gene and thus the competition between dsDNA formation and the sequestering of ssDNA- compare the results to find the optimum level of phage production possible | ||
|- | |- | ||
| VI | | VI | ||
| Phage tail protein (5 copies) | |||
| Add some sort of tag to the gene that is only visible when p6 is outside of the bacteria- thus we would be able to determine when the phage has been secreted | | Add some sort of tag to the gene that is only visible when p6 is outside of the bacteria- thus we would be able to determine when the phage has been secreted | ||
|- | |- | ||
| VII | | VII | ||
| | | Phage head protein (5 copies) | ||
| | | | ||
|- | |- | ||
| VIII | | VIII | ||
| | | Phage coat protein (2700 copies) | ||
| | | | ||
|- | |- | ||
| IX | | IX | ||
| | | Phage head protein (5 copies) | ||
| | | | ||
|- | |- | ||
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|- | |- | ||
| XI | | XI | ||
| | | Assembly | ||
|} | |} | ||
Revision as of 20:54, 12 February 2007
About Me
Name: Dawn Spelke
Major: 20 (Biological Engineering)
Minor: 9 (Brain and Cognitive Sciences)
Class Year: 2009
Email: dspelke AT mit DOT edu
Residence: Burton-Conner
Current Subjects
- 20.209
- 7.05
- 6.00
- 9.59
- 9.65
Research
I'm during a UROP in the Samson Lab/ Center for Environmental Health Sciences. We are studying the effects of alkylating agents on retinal degeneration.
Module 1: Genome Engineering
| protein | function | re-engineering ideas |
|---|---|---|
| I | Assembly | Modifiy the gene so that the channels becomes less selective and thus allow other ions, molecules, and proteins to enter and exit the bacteria- see how this affects the life cycles of both the bacteria and the phage |
| II | Replication of DNA + strand | Alter the gene to make it more active and thus replicate DNA more frequently- see how increased DNA production affects phage growth |
| III | Phage tail protein (5 copies) | Modify the proteins that bind to the bacteria (and thus initiate the F pilus and infection) so that the bacteriophage canbind to and infect other types of bacteria- examine the varied life cycles that result |
| IV | Assembly | Alter the gene in such a way as to destabalize the outer membrane (e.g. no longer detergent-resistant)- test varying environments for phage survival rate |
| V | Binds ssDNA | Vary the activity of the gene and thus the competition between dsDNA formation and the sequestering of ssDNA- compare the results to find the optimum level of phage production possible |
| VI | Phage tail protein (5 copies) | Add some sort of tag to the gene that is only visible when p6 is outside of the bacteria- thus we would be able to determine when the phage has been secreted |
| VII | Phage head protein (5 copies) | |
| VIII | Phage coat protein (2700 copies) | |
| IX | Phage head protein (5 copies) | |
| X | DNA replication | |
| XI | Assembly |
