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| ===20.109 Stuff=== | | ===20.109 Stuff=== |
| [[Dspelke- Module 1: Genome Engineering]] | | [[Dspelke- Module 1: Genome Engineering]] |
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| ===Module 1: Genome Engineering===
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| {| border="1"
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| ! Protein
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| ! Function
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| ! Re-engineering Ideas
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| |-
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| | I
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| | Assembly
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| | Modifiy the gene so that the channels becomes less selective and thus allow other ions, molecules, and proteins to enter and exit the bacteria- see how this affects the life cycles of both the bacteria and the phage
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| |-
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| | II
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| | Replication of DNA + strand
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| | Alter the gene to make it more active and thus replicate DNA more frequently- see how increased DNA production affects phage growth
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| |-
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| | III
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| | Phage tail protein (5 copies)
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| | Modify the proteins that bind to the bacteria (and thus initiate the F pilus and infection) so that the bacteriophage canbind to and infect other types of bacteria- examine the varied life cycles that result
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| |-
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| | IV
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| | Assembly
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| | Alter the gene in such a way as to destabalize the outer membrane (e.g. no longer detergent-resistant)- test varying environments for phage survival rate
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| |-
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| | V
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| | Binds ssDNA
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| | Vary the activity of the gene and thus the competition between dsDNA formation and the sequestering of ssDNA- compare the results to find the optimum level of phage production possible
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| |-
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| | VI
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| | Phage tail protein (5 copies)
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| | Add some sort of tag to the gene that is only visible when p6 is outside of the bacteria- thus we would be able to determine when the phage has been secreted
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| |-
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| | VII
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| | Phage head protein (5 copies)
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| | Alter the gene so that p8 cannot be substituted for p5- see how this affects the phage (e.g. can it still be secreted?)
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| |-
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| | VIII
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| | Phage coat protein (2700 copies)
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| | Add a small protein to the gene that we would like to amplify becuase p8 is synthesized so many times- see if this method works and if yes, what applications could this be used for?
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| |-
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| | IX
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| | Phage head protein (5 copies)
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| | Modify the gene so that it can bind to bacterial surface proteins (like p3 does)- see if this allows the phage to interact with other bacteria (now that both ends can bind)
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| |-
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| | X
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| | DNA replication
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| | Altering this gene will also alter gene 2 so any alteration would affect both genes, so make any number of small modifications - see what interesting phenomena result
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| |-
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| | XI
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| | Assembly
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| | Modify the gene so that it is longer, hopefully resulting in a larger channel- see if this could allow multiple phages to pass through, thus making the channels more effective
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| |}
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About Me
Name: Dawn Spelke
Major: 20 (Biological Engineering)
Minor: 9 (Brain and Cognitive Sciences)
Class Year: 2009
Email: dspelke AT mit DOT edu
Residence: Burton-Conner
Current Subjects
- 20.209
- 7.05
- 6.00
- 9.59
- 9.65
Research
I'm doing a UROP in the Samson Lab/ Center for Environmental Health Sciences. We are studying the effects of alkylating agents on retinal degeneration.
20.109 Stuff
Dspelke- Module 1: Genome Engineering
