User:Dspelke
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About Me
Name: Dawn Spelke
Major: 20 (Biological Engineering)
Minor: 9 (Brain and Cognitive Sciences)
Class Year: 2009
Email: dspelke AT mit DOT edu
Residence: Burton-Conner
Current Subjects
- 20.209
- 7.05
- 6.00
- 9.59
- 9.65
Research
I'm during a UROP in the Samson Lab/ Center for Environmental Health Sciences. We are studying the effects of alkylating agents on retinal degeneration.
Module 1: Genome Engineering
protein | function | re-engineering ideas |
---|---|---|
I | Assembly | Modifiy the gene so that the channels becomes less selective and thus allow other ions, molecules, and proteins to enter and exit the bacteria- see how this affects the life cycles of both the bacteria and the phage |
II | Replication of DNA + strand | Alter the gene to make it more active and thus replicate DNA more frequently- see how increased DNA production affects phage growth |
III | Phage tail protein (5 copies) | Modify the proteins that bind to the bacteria (and thus initiate the F pilus and infection) so that the bacteriophage canbind to and infect other types of bacteria- examine the varied life cycles that result |
IV | Assembly | Alter the gene in such a way as to destabalize the outer membrane (e.g. no longer detergent-resistant)- test varying environments for phage survival rate |
V | Binds ssDNA | Vary the activity of the gene and thus the competition between dsDNA formation and the sequestering of ssDNA- compare the results to find the optimum level of phage production possible |
VI | Phage tail protein (5 copies) | Add some sort of tag to the gene that is only visible when p6 is outside of the bacteria- thus we would be able to determine when the phage has been secreted |
VII | Phage head protein (5 copies) | |
VIII | Phage coat protein (2700 copies) | |
IX | Phage head protein (5 copies) | |
X | DNA replication | |
XI | Assembly |