User:Dstoebel

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Contact Information

Dan Stoebel

Department of Microbiology
Moyne Institute of Preventive Medicine
Trinity College, Dublin
Dublin 2
Ireland

About me

I am interested in the evolution of regulatory networks in bacteria, and how horizontally acquired genes are integrated into those networks. I am currently a post-doctoral fellow with Charles Dorman at Trinity College, Dublin. I did my Ph.D. with Dan Dykhuizen in the Department of Ecology and Evolution at Stony Brook University, graduating in December, 2006. My undergraduate degree is from Pomona College.

Publications

D. M. Stoebel, A. Free, and C. J. Dorman. 2008. Anti-silencing: overcoming H-NS-mediated repression of transcription in Gram-negative enteric bacteria. Microbiology, 154: 2533-2545. Full Text

D. M. Stoebel, A. M. Dean, and D. E. Dykhuizen. 2008. The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products. Genetics, 178:1653-1660. Full Text

D. M. Stoebel. 2005. Lack of evidence for horizontal transfer of the lac operon into Escherichia coli. Molecular Biology and Evolution. 22:683-690. Full Text

M. Feldgarden, D. M. Stoebel, D. Brisson, and D.E. Dykhuizen. 2003. Size doesn't matter: microbial selection experiments address ecological questions. Ecology 84:1679-1687.

Protocols that I use

λ-red mediated recombination

Chloroquine gels

P1 transduction

P22 transduction

Quick and dirty electrocompotent cells

Making Intracellular Salts Medium