User:Easilva/Notebook/Gels with PGE2/2012/08/27

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(Description of bench work and observations)
Current revision (20:21, 28 August 2012) (view source)
(Notes)
 
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* Not sure if we can measure the levels of PGE2 in solution by using a simple assay other than ELISA, including the 660 nm Protein Assay from Pierce... Needs to be figured.
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Gels with PGE2 - Collaboration Main project page
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Description of bench work and observations

  • Briefly discussion with Jen and Scott;
  • Showed to me the data obtain from (n=1) a pilot study using alginate - Idea was to measure the base line in terms of inflammation;
  • It looks that some inflammation has been obtained from the alginate gels;
  • it was discussed that a endotoxin level test could be done from our gels - they might have an extra endotoxin level assay that we can use;
  • it should be useful to get a release profile form the PGE2 gels - needs to have ELISA;

Notes

  • Not sure if we can measure the levels of PGE2 in solution by using a simple assay other than ELISA, including the 660 nm Protein Assay from Pierce... Needs to be figured.



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