User:Easilva/Notebook/OEC isolations/2013/02/24

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(Autocreate 2013/02/24 Entry for User:Easilva/Notebook/OEC_isolations)
Current revision (13:45, 24 February 2013) (view source)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> OEC_20130207F</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==20130224==
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* Insert content here...
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* Cells were feed and passaged - Both cells that were on 1:1 wells (21 Feb and 22 Feb) were passaged to T75 flasks - one normoxia conditions and the the other Hypoxia conditions. Also another, one well from cells, that were passaged before, but some remaining cells stayed behind did proliferated and were again passage for a T25 flask;
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* From normoxia, 5 out of 6 wells, did developed and isolated OECs (on day 17 since isolation);
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*From Hypoxia, 1 out of 6 wells, did developed and isolated OECs (on day 17 since isolation;
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* PW agreed to feed cells while I am out of town;

Current revision

OEC_20130207F Main project page
Previous entry      

20130224

  • Cells were feed and passaged - Both cells that were on 1:1 wells (21 Feb and 22 Feb) were passaged to T75 flasks - one normoxia conditions and the the other Hypoxia conditions. Also another, one well from cells, that were passaged before, but some remaining cells stayed behind did proliferated and were again passage for a T25 flask;
  • From normoxia, 5 out of 6 wells, did developed and isolated OECs (on day 17 since isolation);
  • From Hypoxia, 1 out of 6 wells, did developed and isolated OECs (on day 17 since isolation;
  • PW agreed to feed cells while I am out of town;



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