User:Easilva/Notebook/Sprouting/2012/08/28

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(Autocreate 2012/08/28 Entry for User:Easilva/Notebook/Sprouting)
Current revision (18:52, 28 August 2012) (view source)
(Description of experiments // Details // Protocols // Log)
 
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==Description of experiments // Details // Protocols // Log ==
==Description of experiments // Details // Protocols // Log ==
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* Cells were labeled with Calcein AM;
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* All reagents needed for the sprouting assay were reconstituted and made fresh working solutions (all Aprotinin and Thrombin) were stored at -20˚C;
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* a pilot sprouting assay was initiated with 1 NGF condition and 3 All media conditions. The cells loaded on the beads were labeled with Calcein AM;
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* Priscilla and Vivian were present and also were able to make their own gels;
==Notes==
==Notes==

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Description of experiments // Details // Protocols // Log

  • Cells were labeled with Calcein AM;
  • All reagents needed for the sprouting assay were reconstituted and made fresh working solutions (all Aprotinin and Thrombin) were stored at -20˚C;
  • a pilot sprouting assay was initiated with 1 NGF condition and 3 All media conditions. The cells loaded on the beads were labeled with Calcein AM;
  • Priscilla and Vivian were present and also were able to make their own gels;

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