User:Elad Noor/Notebook/Growth rates of Baceria/2008/08/07
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Experimental plan 1. Grow MG1655 in Arabinose overnight in advance.
2. Mix 19.8 mL of M9 medium, with 20 uL of cells - denote as MA-3 (MG1655 grown on Arabinose, diluted 1:1000 with M9).
3. Prepare a row in a 96 plate:
1. Put 150 uL of 20% Arabinose in the first well
2. Put 100 uL of MA-3 in all of the other 11 wells in the row
1. For i = 1..11
1. Pick up 50 uL (third) out of well i
2. Insert into well i+1 and mix
4. Fill every well in a new 96 plate with 145 uL of MA-3
5. Move 7.5 uL of the row in plate 1 into each row in plate 2 (i.e. dilute the arabinose with MA-3 by 1:20.33)
Now we get, 0.98% (= 20% / 20.33) Arabinose inside 150 uL of MA-3 for the first column, 0.33% in the second column, 0.11% in the third, and so on...
6. Add 100 uL of Oil in each well.
7. Grow in 37 degrees and measure the OD during 24 hours (300 times).
Results file: File:Mg1655+arabinose 07-08-08.xls | |
