User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/06: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Zein Protein Film</span>
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==Entry Title==


==Objective==


==Objective==
To make a zein protein film to prepare for a later experiment.
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==Description==
==Description==
# Add experimental record here. Include what, how, and why...
 
# Make zein protein solution (10% w/v) in aqueous ethanol (90% v/v) and glycerol (30% on zein weight basis) as plasticizer.
# Place 5 g of filmogenic solution on 9 cm diameter Petri dish. Swirl to coat the bottom of the dish.
# Place Petri dish without lid on a level surface (checked with spirit level) in an oven at 40°C for 24 hr.


==Data==
==Data==
* Add data and results here...
 
<center>
[[Image:Petri1.jpg|300px]]
</center>
*The film appeared yellowish and shiny on observation.
 
<center>
[[Image:Petri11.jpg|300px]]
</center>
*The film was discovered to be very brittle when an attempt was made to remove it from the Petri dish. It crumbled and broke when under any kind of stress.
 
<center>
[[Image:Zein_protein_film.JPG|300px]]
</center>
*Under the microscope, the film appeared speckled with what is most likely zein protein.


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
The protein film was very brittle and broke easily. This is most likely due to too low a volume of glycerol added, as the viscous glycerol was very difficult to pipette into the reaction mixture. In a future experiment, more glycerol needs to be used to ensure a flexible protein film.


[[Category:Course]]
[[Category:Course]]

Revision as of 11:16, 14 September 2011

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Objective

To make a zein protein film to prepare for a later experiment.

Description

  1. Make zein protein solution (10% w/v) in aqueous ethanol (90% v/v) and glycerol (30% on zein weight basis) as plasticizer.
  2. Place 5 g of filmogenic solution on 9 cm diameter Petri dish. Swirl to coat the bottom of the dish.
  3. Place Petri dish without lid on a level surface (checked with spirit level) in an oven at 40°C for 24 hr.

Data

  • The film appeared yellowish and shiny on observation.

  • The film was discovered to be very brittle when an attempt was made to remove it from the Petri dish. It crumbled and broke when under any kind of stress.

  • Under the microscope, the film appeared speckled with what is most likely zein protein.

Notes

The protein film was very brittle and broke easily. This is most likely due to too low a volume of glycerol added, as the viscous glycerol was very difficult to pipette into the reaction mixture. In a future experiment, more glycerol needs to be used to ensure a flexible protein film.



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