User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/06

From OpenWetWare
Jump to navigationJump to search
Zein Protein Film <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objective

To make a zein protein film to prepare for a later experiment.

Description

  1. Make zein protein solution (10% w/v) in aqueous ethanol (90% v/v) and glycerol (30% on zein weight basis) as plasticizer.
  2. Place 5 g of filmogenic solution on 9 cm diameter Petri dish. Swirl to coat the bottom of the dish.
  3. Place Petri dish without lid on a lever surface (checked with spirit level) in an oven at 40°C for 24 hr.

Data

  • Add data and results here...

Notes

The protein film was very brittle and broke easily. This is most likely due to too low a volume of glycerol added, as the viscous glycerol was very difficult to pipette into the reaction mixture. In a future experiment, more glycerol needs to be used to ensure a flexible protein film.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/09/06&oldid=534983"