User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/20: Difference between revisions
From OpenWetWare
(→Notes) |
(→Data) |
||
Line 28: | Line 28: | ||
==Data== | ==Data== | ||
Forward Primer: | Primer determined using http://www.basic.northwestern.edu/biotools/oligocalc.html | ||
*Forward Primer: | |||
::GAT AAG GAT GAC GAT AAG <b>TGT</b> CGA TGG GGA TCC GAA TTC GCC | ::GAT AAG GAT GAC GAT AAG <b>TGT</b> CGA TGG GGA TCC GAA TTC GCC | ||
Reverse Primer: | *Reverse Primer: | ||
::GGC GAA TTC GGA TCC CCA TCG <b>ACA</b> CTT ATC GTC ATC CTT ATC | ::GGC GAA TTC GGA TCC CCA TCG <b>ACA</b> CTT ATC GTC ATC CTT ATC | ||
(Mutation in bold) | ::(Mutation in bold) | ||
39bp, 51% GC content, T<sub>m</sub>=78.8°C (salt adjusted). | ::39bp, 51% GC content, T<sub>m</sub>=78.8°C (salt adjusted). | ||
==Notes== | ==Notes== |
Revision as of 11:10, 20 September 2011
Mutation and Amplification of GFP | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo perform a PCR reaction to mutate Green Fluorescent Proten (GFP) to contain a cysteine amino acid just after the enterokinase cleavage site. Description
DataPrimer determined using http://www.basic.northwestern.edu/biotools/oligocalc.html
NotesPrimers must have a melting point Tm ≥ 78°C, a GC content ≥ 40%, and be between 25-45 bases in length.
|