User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/20
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Mutation and Amplification of GFP | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo perform a PCR reaction to mutate Green Fluorescent Proten (GFP) to contain a cysteine amino acid just after the enterokinase cleavage site. Description
DataForward Primer:
Reverse Primer:
(Mutation in bold) 39bp, 51% GC content, Tm=78.8°C (salt adjusted), NotesUse categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
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