User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/20
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Mutation and Amplification of GFP | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo perform a PCR reaction to mutate Green Fluorescent Proten (GFP) to contain a cysteine amino acid just after the enterokinase cleavage site.
Description
DataPrimer determined using http://www.basic.northwestern.edu/biotools/oligocalc.html
NotesPrimers must have a melting point Tm ≥ 78°C, a GC content ≥ 40%, and be between 25-45 bases in length. Sequence of GFP vector provided by [1]
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