User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/21: Difference between revisions
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# Prepare a ladder containing DNA, glycerol, and gel loading dye. | # Prepare a ladder containing DNA, glycerol, and gel loading dye. | ||
# Load DNA solutions and run the gel at 80 V for 40 min. | # Load DNA solutions and run the gel at 80 V for 40 min. | ||
# Remove gel and place in TAE and ethidium bromide (EtBr)solution, mix for 15 min. | # Remove gel and place in TAE and ethidium bromide (EtBr) solution, mix for 15 min. | ||
# Rinse gel with TAE for 5 min. | # Rinse gel with TAE for 5 min. | ||
Revision as of 12:05, 21 September 2011
Gel Electrophoresis of GFP | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo determine whether or not GFP is present in solutions made on 09/20/11 using agarose gel electrophoresis. Description
DataNotesGel ladder: 500 bp, 1000 bp, 2000 bp, 3000 bp, 4000 bp, 5000 bp, 6000 bp, 7000 bp, 8000 bp, 9000 bp, 10000 bp An optimum gel would have used 1.5% agarose.
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