User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/21: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Gel Electrophoresis of | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Gel Electrophoresis of PCR Product</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Objective== | ==Objective== | ||
To determine whether or not | To determine whether or not the PCR performed on [http://openwetware.org/wiki/User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/09/20 09/20/11] of the DNA coding for a mutant GFP was successful using agarose gel electrophoresis. | ||
==Description== | ==Description== | ||
# Make a 1% agarose gel by adding 0.25 g of 0.01 g/mL of agarose to 25 mL of 1x tris base, acetic acid, and ethylenediaminetetraacetic acid (TAE) buffer and heating the mixture in the microwave for 40 s. | |||
# Mix 1 μL of 6x gel loading dye with 5 μL of the DNA sample. | |||
# Prepare a ladder containing DNA, glycerol, and gel loading dye. | |||
# Load DNA solutions and run the gel at 80 V for 40 min. | |||
# Remove gel and place in TAE and ethidium bromide (EtBr) solution, mix for 15 min. | |||
# Rinse gel with TAE for 5 min. | |||
==Data== | ==Data== | ||
A photo of the electrophoresis gel is displayed below: | |||
<center>[[image:DNA gel 092111 003.jpg]]</center> | |||
The first column is the ladder. The next columns are groups 1, 2, 3, 4, and 5. | |||
==Notes== | ==Notes== | ||
Gel ladder: | |||
500 bp, 1000 bp, 2000 bp, 3000 bp, 4000 bp, 5000 bp, 6000 bp, 7000 bp, 8000 bp, 9000 bp, 10000 bp | |||
An optimum gel would have used 1.5% agarose. | |||
Revision as of 12:07, 4 October 2011
 Gel Electrophoresis of PCR Product
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
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ObjectiveTo determine whether or not the PCR performed on 09/20/11 of the DNA coding for a mutant GFP was successful using agarose gel electrophoresis. Description
DataA photo of the electrophoresis gel is displayed below:  The first column is the ladder. The next columns are groups 1, 2, 3, 4, and 5. NotesGel ladder: 500 bp, 1000 bp, 2000 bp, 3000 bp, 4000 bp, 5000 bp, 6000 bp, 7000 bp, 8000 bp, 9000 bp, 10000 bp An optimum gel would have used 1.5% agarose.
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