User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/21

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Objective

To determine whether or not GFP is present in solutions made on 09/20/11 using agarose gel electrophoresis.

Description

  1. Make a 1% agarose gel by adding 0.25 g of 0.01 g/mL of agarose to 25 mL of 1x tris base, acetic acid, and ethylenediaminetetraacetic acid (TAE) buffer and heating the mixture in the microwave for 40 s.
  2. Mix 1 μL of 6x gel loading dye with 5 μL of the DNA sample.
  3. Prepare a ladder containing DNA, glycerol, and gel loading dye.

Data

Notes

An optimum gel would have used 1.5% agarose.




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