User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/27: Difference between revisions
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<b>AuNP III</b> | <b>AuNP III</b> | ||
# Combine 10 mL of 50 mM acetate buffer (pH 5.46) with | # Combine 10 mL of 50 mM acetate buffer (pH 5.46) with 0.974 mL of 0.0015 mM BSA and 0.581 mL of 0.25 mM HAuCl<sub>4</sub> in a capped test tube at room temperature. | ||
# Take UV vis spectra (200 nm to 800 nm) of a sample of reaction mixture at 70°C every 30 min. | |||
# Take UV vis spectra (200 nm to 800 nm) of | # Place reaction mixture in an oven at 80°C for 2.5 hr under thermostatic conditions. | ||
# Place reaction | |||
# After 2.5 hr, remove from oven and cool to room temperature; leave sitting overnight. | # After 2.5 hr, remove from oven and cool to room temperature; leave sitting overnight. | ||
Revision as of 09:15, 28 September 2011
AuNP III, Expression, DNA Transformation | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAuNP III To synthesize gold nanoparticles (Au NP) using the procedure developed here:
http://pubs.acs.org/doi/abs/10.1021/jp110296y
To begin culturing bacteria to express inteins.
To transform DNA coding for mutant GFP (mutated on 09/20/11) into Novablue cells. DescriptionAuNP III
Starter Culture Media, Protein Expression
DataNotesAuNP solution turned purple while in the oven, indicating that AuNPs were actually formed.
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