User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/27: Difference between revisions
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==Data== | ==Data== | ||
The absorbance values for the AuNP solution were subtracted from the absorbance values for a blank acetate buffer to create a set of corrected absorbance values. The corrected absorbance values at 550 nm were graphed against time. The graph shows a positive linear relationship, indicating that AuNPs were formed in solution. | |||
[[Image:Absorbance vs | <center>[[Image:Absorbance vs time 9-27-11.jpg|600px]]</center> | ||
[[Image:Absorbance vs wavelength magnified 9-27-11.jpg]] | In addition, a second graph showing corrected absorbance against wavelength was created, to show the absorbance levels across all of the wavelengths measured by the UV-Vis. | ||
<center>[[Image:Absorbance vs wavelength 9-27-11.jpg|600px]]</center> | |||
A magnified version of this graph shows that absorbance values at around 550 nm increased over time, indicating the creation of AuNPs. | |||
<center>[[Image:Absorbance vs wavelength magnified 9-27-11.jpg|600px]]</center> | |||
==Notes== | ==Notes== |
Revision as of 10:17, 28 September 2011
AuNP III, Expression, DNA Transformation | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAuNP III To synthesize gold nanoparticles (Au NP) using the procedure developed here:
http://pubs.acs.org/doi/abs/10.1021/jp110296y
To begin culturing bacteria to express inteins.
To transform DNA coding for mutant GFP (mutated on 09/20/11) into Novablue cells. DescriptionAuNP III
Starter Culture Media, Protein Expression
DataThe absorbance values for the AuNP solution were subtracted from the absorbance values for a blank acetate buffer to create a set of corrected absorbance values. The corrected absorbance values at 550 nm were graphed against time. The graph shows a positive linear relationship, indicating that AuNPs were formed in solution. In addition, a second graph showing corrected absorbance against wavelength was created, to show the absorbance levels across all of the wavelengths measured by the UV-Vis. A magnified version of this graph shows that absorbance values at around 550 nm increased over time, indicating the creation of AuNPs. NotesAuNP solution turned purple while in the oven, indicating that AuNPs were actually formed.
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