User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/05: Difference between revisions

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<center>[[Image:Integral of intensity vs concentration.jpg|600px]]</center>
<center>[[Image:Integral of intensity vs concentration.jpg|600px]]</center>
Concentrations of MBP solutions:
Blank: 50 mM Tris (7.55 pH)
A: 1397 μM MBP stock + 1603 μM Tris
B: 300 μM A + 2700 μM Tris
C: 300 μM B + 2700 μM Tris
D: 300 μM C + 2700 μM Tris
E: 300 μM D + 2700 μM Tris
<center>[[Image:Intensity over concentration vs wavelength a 10-5-11.jpg|600px]]</center>
<center>[[Image:Intensity over concentration vs wavelength b 10-5-11.jpg|600px]]</center>
<center>[[Image:Intensity over concentration vs wavelength c 10-5-11.jpg|600px]]</center>
<center>[[Image:Intensity over concentration vs wavelength d 10-5-11.jpg|600px]]</center>
<center>[[Image:Intensity over concentration vs wavelength e 10-5-11.jpg|600px]]</center>


==Notes==
==Notes==

Revision as of 14:29, 10 October 2011

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Objective

Fluorimeter Sensitivity II

To continue the experiment on 10/04/11 to determine the sensitivity of a fluorimeter using standard concentrations of Ru(bpy)(phen) and determine the fluorescence of MBP as a function of concentration.


DNA Purification

To purify the DNA made by cells cultured on 09/27/11.

Description

Fluorimeter Sensitivity II

  1. Integrate the intensity values found on 10/05/11 and graph them against concentration.


DNA Purification

The DNA purification protocol was adapted from the Wizard Plus SV Minpreps DNA Purification System vacuum protocol found here:

http://www.promega.com/~/media/Files/Resources/ProtCards/Wizard%20Plus%20SV%20Minipreps%20DNA%20Purification%20System%20Quick%20Protocol.ashx

Data

Integrated intensity of Ru(bpy)(phen):


20 μM: 1432.805647

40 μM: 2001.315233

60 μM: 2237.400074

80 μM: 2294.581483

100 μM: 2286.935912


This graph shows the integral of fluorescence intensity vs. the concentration of Ru(bpy)(phen). The graph begins to level off at 60 μM, indicating that the Ru(bpy)(phen) is emitting too much light for the fluorimeter at around that point. This is consistent with the findings on 10/04/11



Concentrations of MBP solutions:


Blank: 50 mM Tris (7.55 pH)

A: 1397 μM MBP stock + 1603 μM Tris

B: 300 μM A + 2700 μM Tris

C: 300 μM B + 2700 μM Tris

D: 300 μM C + 2700 μM Tris

E: 300 μM D + 2700 μM Tris

Notes




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