User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/05: Difference between revisions
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==Data== | ==Data== | ||
This graph shows the integral of fluorescence intensity vs. the concentration of Ru(bpy)(phen). The graph begins to level off at 60 μM, indicating that the Ru(bpy)(phen) is emitting too much light for the fluorimeter at around that point. This is consistent with the findings on [http://openwetware.org/wiki/User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/04 10/04/11] | This graph shows the integral of fluorescence intensity vs. the concentration of Ru(bpy)(phen). The graph begins to level off at 60 μM, indicating that the Ru(bpy)(phen) is emitting too much light for the fluorimeter at around that point. This is consistent with the findings on [http://openwetware.org/wiki/User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/04 10/04/11] | ||
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<center>[[ | <center>[[Molar_absorptivity_vs_wavelength_10-5-11.jpg|600px]]</center> | ||
Calculated concentrations of MBP dilutions: | Calculated concentrations of MBP dilutions: | ||
* A 1.6700×10<sup>-6</sup> | * A 1.6700×10<sup>-6</sup> M | ||
* B 3.0895×10<sup>-7</sup> | * B 3.0895×10<sup>-7</sup> M | ||
* C 2.3911×10<sup>-8</sup> | * C 2.3911×10<sup>-8</sup> M | ||
* D 5.6788×10<sup>-8</sup> | * D 5.6788×10<sup>-8</sup> M | ||
* E 8.9665×10<sup>-9</sup> | * E 8.9665×10<sup>-9</sup> M | ||
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==Notes== | ==Notes== | ||
Integrated intensity of Ru(bpy)(phen): | |||
*20 μM: 1432.805647 | |||
*40 μM: 2001.315233 | |||
*60 μM: 2237.400074 | |||
*80 μM: 2294.581483 | |||
*100 μM: 2286.935912 | |||
Revision as of 13:42, 30 November 2011
Fluorimeter Sensitivity II, DNA Purification | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveFluorimeter Sensitivity II To continue the experiment on 10/04/11 to determine the sensitivity of a fluorimeter using standard concentrations of Ru(bpy)(phen) and determine the fluorescence of MBP as a function of concentration.
To purify the DNA made by cells cultured on 09/27/11. DescriptionFluorimeter Sensitivity II
DNA Purification The DNA purification protocol was adapted from the Wizard Plus SV Minpreps DNA Purification System vacuum protocol found here: DataThis graph shows the integral of fluorescence intensity vs. the concentration of Ru(bpy)(phen). The graph begins to level off at 60 μM, indicating that the Ru(bpy)(phen) is emitting too much light for the fluorimeter at around that point. This is consistent with the findings on 10/04/11
NotesIntegrated intensity of Ru(bpy)(phen):
|