User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/12: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Conductivity of AuNP II</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Conductivity of AuNP II, DNA Sequencing</span> | ||
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==Objective== | |||
<b>Conductivity of AuNP II</b> | |||
To repeat the experiment on [http://openwetware.org/wiki/User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/11 10/11/11] and make electrodes to test the conductivity of MBP protein fibers containing AuNPs using a four point probe. | |||
<b>DNA Sequencing</b> | |||
To | To compare the mutant GFP DNA sequence purified on [http://openwetware.org/wiki/User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/05 10/05/11] to the wild type GFP DNA sequence to determine if the mutation is present. | ||
==Description== | ==Description== | ||
<b>Conductivity of AuNP II</b> | |||
# Glue Pd wire onto Pd foil using conductive epoxy. | # Glue Pd wire onto Pd foil using conductive epoxy. | ||
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# Repeat with 3 more wires/foils. | # Repeat with 3 more wires/foils. | ||
# Glue wires/foils to Teflon plate with Instant Krazy Glue. | # Glue wires/foils to Teflon plate with Instant Krazy Glue. | ||
# When dry, plate AuNP-containing protein fibers onto the Teflon across the Pd foils. | |||
# Allow the solvent to evaporate overnight. | |||
<b>DNA Sequencing</b> | |||
# Compare the obtained DNA sequence of GFP to the wild type DNA sequence from [https://docs.google.com/document/pub?id=1q9liaSkDZ_35N6F_ztcXqKOu6g1w6qxqpCQ-tD8zOVM Invitrogen] | |||
==Data== | ==Data== | ||
<b>Conductivity of AuNP II</b> | |||
Areas of Pd foil used, from left to right: 35.20 mm<sup>2</sup>, 41.16 mm<sup>2</sup>, 40.18 mm<sup>2</sup>, 38.64 mm<sup>2</sup>. | Areas of Pd foil used, from left to right: 35.20 mm<sup>2</sup>, 41.16 mm<sup>2</sup>, 40.18 mm<sup>2</sup>, 38.64 mm<sup>2</sup>. | ||
<b>DNA Sequencing</b> | |||
Obtained DNA sequence of mutant GFP, mutation site bold and underlined: | |||
NNNNNNNNNNNTTTGTTNNACTTTAAGAAGGAGNTATACATATGCGGGGTTCTCATCATCATCATCATCATGGTATGGCTAGCAT | |||
GACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAG<b><u>GAT</u></b>CGATGGGGATCCGAATTCGCCACCATGGTGAGC | |||
AAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG | |||
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCAC | |||
CCTCGTGACCACCTTGACCTACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCC | |||
ATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGG | |||
GCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAA | |||
CTACAACAGCCACAAGGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGACCCGCCACAACATCGAG | |||
GACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACC | |||
TGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGAT | |||
CACTCTCGGCATGGACGAGCTGTACAAGTAACTCGAGAAGCTTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCT | |||
GCTGCCACCGCTGANCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACT | |||
The DNA sequence at the mutation site is identical to the wild type sequence. Therefore, the DNA was not mutated as intended. | |||
==Notes== | ==Notes== | ||
<b>Conductivity of AuNP II</b> | |||
Electrodes made on [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/11|10/11/11]] were not functional because the conductive epoxy used did not successfully glue the Pd foil and wire to the Teflon surface. | |||
<b>DNA Sequencing</b> | |||
It was determined that the primers used to create the mutation in the GFP DNA sequence actually coded for the wild type GFP sequence. Thus, no mutation was possible. | |||
[[Category:Course]] | [[Category:Course]] | ||
Revision as of 10:38, 1 November 2011
 Conductivity of AuNP II, DNA Sequencing
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ObjectiveConductivity of AuNP II To repeat the experiment on 10/11/11 and make electrodes to test the conductivity of MBP protein fibers containing AuNPs using a four point probe.
To compare the mutant GFP DNA sequence purified on 10/05/11 to the wild type GFP DNA sequence to determine if the mutation is present. DescriptionConductivity of AuNP II
DNA Sequencing
DataConductivity of AuNP II Areas of Pd foil used, from left to right: 35.20 mm2, 41.16 mm2, 40.18 mm2, 38.64 mm2.
Obtained DNA sequence of mutant GFP, mutation site bold and underlined: NNNNNNNNNNNTTTGTTNNACTTTAAGAAGGAGNTATACATATGCGGGGTTCTCATCATCATCATCATCATGGTATGGCTAGCAT GACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCGATGGGGATCCGAATTCGCCACCATGGTGAGC AAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCAC CCTCGTGACCACCTTGACCTACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCC ATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGG GCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAA CTACAACAGCCACAAGGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGACCCGCCACAACATCGAG GACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACC TGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGAT CACTCTCGGCATGGACGAGCTGTACAAGTAACTCGAGAAGCTTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCT GCTGCCACCGCTGANCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACT The DNA sequence at the mutation site is identical to the wild type sequence. Therefore, the DNA was not mutated as intended. NotesConductivity of AuNP II Electrodes made on 10/11/11 were not functional because the conductive epoxy used did not successfully glue the Pd foil and wire to the Teflon surface.
It was determined that the primers used to create the mutation in the GFP DNA sequence actually coded for the wild type GFP sequence. Thus, no mutation was possible.
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