User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/12

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Conductivity of AuNP II, DNA Sequencing <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

Conductivity of AuNP II

To repeat the experiment on 10/11/11 and make electrodes to test conductivity of MBP protein fibers containing AuNPs using a four point probe.


DNA Sequencing

To compare the mutant GFP DNA sequence purified on 10/05/11 to the wild type GFP DNA sequence to determine if the mutation is present.

Description

Conductivity of AuNP II

  1. Glue Pd wire onto Pd foil using conductive epoxy.
  2. Heat with heat gun until epoxy dries.
  3. Repeat with 3 more wires/foils.
  4. Glue wires/foils to Teflon plate with Instant Krazy Glue.

DNA Sequencing

  1. Compare the obtained DNA sequence of GFP to the wild type DNA sequence from Invitrogen

Data

Conductivity of AuNP II

Areas of Pd foil used, from left to right: 35.20 mm2, 41.16 mm2, 40.18 mm2, 38.64 mm2.


DNA Sequencing

Obtained DNA sequence of mutant GFP, mutation site bold and underlined:

NNNNNNNNNNNTTTGTTNNACTTTAAGAAGGAGNTATACATATGCGGGGTTCTCATCATCATCATCATCATGGTATGGCTAGCAT GACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCGATGGGGATCCGAATTCGCCACCATGGTGAGC AAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCAC CCTCGTGACCACCTTGACCTACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCC ATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGG GCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAA CTACAACAGCCACAAGGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGACCCGCCACAACATCGAG GACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACC TGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGAT CACTCTCGGCATGGACGAGCTGTACAAGTAACTCGAGAAGCTTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCT GCTGCCACCGCTGANCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACT

The DNA sequence at the mutation site is identical to the wild type sequence. Therefore, the DNA was not mutated as intended.

Notes




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