User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/18: Difference between revisions

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# Vortex to mix.
# Vortex to mix.
# Wrap reaction tube in foil and allow reaction to proceed for 2 hr.
# Wrap reaction tube in foil and allow reaction to proceed for 2 hr.
# Add 100 μL of dithiothreitol (DTT)


==Data==
==Data==

Revision as of 11:17, 18 October 2011

Conductivity of AuNP III, Fluorophore <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

Conductivity of AuNP III

To run the conductive plates made on 10/12/11 to determine the conductivity of AuNP.


Fluorophore

To make a labeled cysteine fluorophore.

Description

Conductivity of AuNP III


Fluorophore

  1. Combine 2 mg of 5-iodoacetamido fluorescein (5-IAF) with excess cysteine (0.39 mg) in 3.95 mL of 50 mM Tris buffer (pH 7.5).
  2. Vortex to mix.
  3. Wrap reaction tube in foil and allow reaction to proceed for 2 hr.
  4. Add 100 μL of dithiothreitol (DTT)

Data

Notes

Fluorophore and references used in this experiment:

http://www.markergene.com/ProductDetails.php/M0638




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