User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/18: Difference between revisions
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|style="background-color: # | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Conductivity of AuNP III, Fluorophore</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |||
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< | ==Objective== | ||
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<b>Conductivity of AuNP III</b> | |||
To run the conductive plates made on [http://openwetware.org/wiki/User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/12 10/12/11] to determine the conductivity of AuNP. | |||
<b>Fluorophore</b> | |||
To make a labeled cysteine fluorophore. | |||
==Description== | ==Description== | ||
<b>Fluorophore</b> | |||
# Combine 2 mg of 5-iodoacetamido fluorescein (5-IAF) with excess cysteine (0.39 mg) in 3.95 mL of 50 mM Tris buffer (pH 7.5). | |||
# Vortex to mix. | |||
# Wrap reaction tube in foil and allow reaction to proceed for 2 hr. | |||
# Add 100 μL of dithiothreitol (DTT) | |||
==Data== | ==Data== | ||
<b>Conductivity of AuNP II</b> | |||
The Pd plates broke off of the Teflon plate, so the test was not conducted. | |||
==Notes== | ==Notes== | ||
Fluorophore and references used in this experiment: | |||
http://www.markergene.com/ProductDetails.php/M0638 | |||
[[Category:Course]] | [[Category:Course]] | ||
[[Category:Miscellaneous]] | [[Category:Miscellaneous]] | ||
Revision as of 10:16, 19 October 2011
 Conductivity of AuNP III, Fluorophore
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ObjectiveConductivity of AuNP III To run the conductive plates made on 10/12/11 to determine the conductivity of AuNP.
To make a labeled cysteine fluorophore. DescriptionFluorophore
DataConductivity of AuNP II The Pd plates broke off of the Teflon plate, so the test was not conducted. NotesFluorophore and references used in this experiment: http://www.markergene.com/ProductDetails.php/M0638
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