User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/25: Difference between revisions
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# Run SDS-PAGE | # Run SDS-PAGE | ||
# Stain gels with Coomassie Brilliant Blue R250 solution | # Stain gels with Coomassie Brilliant Blue R250 solution | ||
## Dissolve 0. | ## Dissolve 0.2555 g Coomassie Brilliant Blue R250 in 90 mL of methanol:water (1:1 v/v) and 10 mL of glacial acetic acid. | ||
## Filter solution through a Whatman No. 1 filter. | ## Filter solution through a Whatman No. 1 filter. | ||
# Rinse gels overnight. | # Rinse gels overnight. |
Revision as of 10:59, 25 October 2011
Protein Gel | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo run a protein gel to determine whether or not MBP-intein fusion proteins are being expressed in cells frozen on 09/28/11. Description
DataNotes
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