User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/25: Difference between revisions
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## Dissolve 0.2555 g Coomassie Brilliant Blue R250 in 90 mL of methanol:water (1:1 v/v) and 10 mL of glacial acetic acid. | ## Dissolve 0.2555 g Coomassie Brilliant Blue R250 in 90 mL of methanol:water (1:1 v/v) and 10 mL of glacial acetic acid. | ||
## Filter solution through a Whatman No. 1 filter. | ## Filter solution through a Whatman No. 1 filter. | ||
# | # Place on shaker overnight. | ||
==Data== | ==Data== |
Revision as of 09:25, 26 October 2011
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ObjectiveTo run an SDS-PAGE to determine whether or not MBP-intein fusion proteins are being expressed cells comparable to the cells frozen on 09/28/11. Description
DataResults will be collected on 10/25/11. NotesProtein solutions used were from group 2:
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