User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/26: Difference between revisions
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==Description== | ==Description== | ||
<b>AuNP VI</b> | |||
# Combine 2 mL HAuCl<sub>4</sub>, 1 mL BSA, and 7 mL of water in a test tube. | |||
# Combine 1 mL BSA and 9 mL of water in a separate test tube. | |||
# Place test tubes in an oven at 80°C for 30 min. | |||
# Remove test tubes from oven for 10 min. | |||
# Repeat steps 3 and 4 for 3.5 hr. | |||
<b>SDS-PAGE II</b> | |||
# Empty Coomassie Brilliant Blue R250 stain from gels. | |||
# Add destaining solution. | |||
# Place gels on shaker for 3.5 hr. | |||
# Dump out destaining solution. | |||
# Add more destaining solution. | |||
# Place on shaker overnight. | |||
==Data== | ==Data== |
Revision as of 09:38, 26 October 2011
AuNP VI, SDS-PAGE II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAuNP VI To synthesize AuNP using the procedure developed here:
http://pubs.acs.org/doi/abs/10.1021/jp110296y
To run an SDS-PAGE to determine whether or not MBP-intein fusion proteins are being expressed cells comparable to the cells frozen on 09/28/11 and to continue the experiment on 10/25/11. DescriptionAuNP VI
DataNotesProtein solutions used were from group 2:
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