User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/01: Difference between revisions
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In addition, to repeat the experiment performed on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2011/08/31|08/31/11]], [[User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/07|09/07/11]], [[User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/27|09/27/11]], [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/09/28|09/28/11]], [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/19|10/19/11]], and [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/26|10/26/11]]. | In addition, to repeat the experiment performed on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2011/08/31|08/31/11]], [[User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/07|09/07/11]], [[User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/27|09/27/11]], [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/09/28|09/28/11]], [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/19|10/19/11]], and [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/26|10/26/11]]. | ||
<b>Mutation and Amplification of GFP II</b> | |||
To perform a PCR reaction to mutate green fluorescent protein (GFP) to contain a cysteine amino acid just after the enterokinase cleavage site. | |||
In addition, to repeat the experiment performed on [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/09/20|09/20/11]]. | |||
Procedure derived from [http://kirschner.med.harvard.edu/files/protocols/Stratagene_quickchangepdf.pdf|Stratagene QuikChange® Site-Directed Mutagenesis Kit] | |||
==Description== | ==Description== |
Revision as of 10:22, 1 November 2011
AuNP VII, Mutation and Amplification of GFP II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAuNP VII To synthesize AuNP using the procedure developed here:
http://pubs.acs.org/doi/abs/10.1021/jp110296y
To perform a PCR reaction to mutate green fluorescent protein (GFP) to contain a cysteine amino acid just after the enterokinase cleavage site. In addition, to repeat the experiment performed on 09/20/11.
DescriptionAuNP VII
DataNotes
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