User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/02: Difference between revisions
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<b>AuNP VIII</b> | <b>AuNP VIII</b> | ||
# Combine 8 mL of water, 1 mL HAuCl<sub>4</sub> (2.9 mM), and 1 mL BSA (1.55 μM) in that order | # Combine 8 mL of water, 1 mL HAuCl<sub>4</sub> (2.9 mM), and 1 mL BSA (1.55 μM) in that order 3 test tubes. | ||
# Mix one test tube. | |||
# Place test tubes in an oven at 80°C for 30 min. | # Place test tubes in an oven at 80°C for 30 min. | ||
# Remove test tubes from oven for 10 min. | # Remove 2 test tubes from oven for 10 min, leave one in the oven. | ||
# Repeat steps | # Repeat steps 3 and 4 for 3.5 hr. | ||
Revision as of 10:51, 2 November 2011
AuNP VIII, DNA Transformation II, Gel II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAuNP VIII To synthesize AuNP using the procedure developed here:
http://pubs.acs.org/doi/abs/10.1021/jp110296y
To transform DNA coding for mutant GFP (mutated on 11/01/11) into Novablue cells.
To determine whether or not the PCR performed on 11/01/11 of the DNA coding for a mutant GFP was successful using agarose gel electrophoresis.
DescriptionAuNP VIII
DataNotesAuNP VIII New HAuCl4 was used to make the nanoparticle solution.
Order of DNA gel: Ladder|Tamra 1|Tamra 2|Group 1|Group 2|Group 3|Group 4|Group 5
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