User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/08: Difference between revisions

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# Sonicate cells: 30 s on, 30 s off 3 times.
# Sonicate cells: 30 s on, 30 s off 3 times.
# Centrifuge at 17000 rpm for 1.5 hr.
# Centrifuge at 17000 rpm for 1.5 hr.
# Run dialysis over night on supernatant.


==Data==
==Data==

Revision as of 13:33, 8 November 2011

Purification <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To purify the proteins expressed in cells frozen on 09/29/11.

Description

  1. Make solutions for tomorrow.
    • 1 L 50 mM Tris (pH 9.02)
    • 1 L 50 mM Tris + 1 M NaCl (pH 9.05)
    • 2 batches of 4 L 50 mM Tris (pH 9.00)
  2. Filter both 1 L solutions.
  3. Defrost cells from 09/28/11 in a water bath.
  4. Sonicate cells: 30 s on, 30 s off 3 times.
  5. Centrifuge at 17000 rpm for 1.5 hr.
  6. Run dialysis over night on supernatant.

Data

Notes



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