User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/08: Difference between revisions
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==Objective== | ==Objective== | ||
To purify the proteins expressed in cells frozen on [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/09/28|09/28/11]]. | |||
==Description== | ==Description== | ||
# Make solutions for tomorrow. | |||
#* 1 L 50 mM Tris (pH 9.02) | |||
#* 1 L 50 mM Tris + 1 M NaCl (pH 9.05) | |||
#* 2 batches of 4 L 50 mM Tris (pH 9.00) | |||
# Filter both 1 L solutions. | |||
# Defrost cells from [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/09/28|09/28/11]] in a water bath. | |||
# Sonicate cells: 30 s on, 30 s off 3 times. | |||
# Centrifuge at 17000 rpm for 1.5 hr. | |||
# Run dialysis overnight on supernatant in the 2 batches of 4L of 50 mM Tris (pH 9). | |||
==Data== | ==Data== |
Revision as of 10:18, 9 November 2011
Purification | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo purify the proteins expressed in cells frozen on 09/28/11. Description
DataNotes
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