User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/08: Difference between revisions

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==Objective==
==Objective==


To purify the proteins expressed in cells frozen on [User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/09/28|09/29/11].
To purify the proteins expressed in cells frozen on [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/09/28|09/28/11]].


==Description==
==Description==


# Make solutions for tomorrow.
#* 1 L 50 mM Tris (pH 9.02)
#* 1 L 50 mM Tris + 1 M NaCl (pH 9.05)
#* 2 batches of 4 L 50 mM Tris (pH 9.00)
# Filter both 1 L solutions.
# Defrost cells from [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/09/28|09/28/11]] in a water bath.
# Sonicate cells: 30 s on, 30 s off 3 times.
# Centrifuge at 17000 rpm for 1.5 hr.
# Run dialysis overnight on supernatant in the 2 batches of 4L of 50 mM Tris (pH 9).


==Data==
==Data==

Revision as of 10:18, 9 November 2011

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Objective

To purify the proteins expressed in cells frozen on 09/28/11.

Description

  1. Make solutions for tomorrow.
    • 1 L 50 mM Tris (pH 9.02)
    • 1 L 50 mM Tris + 1 M NaCl (pH 9.05)
    • 2 batches of 4 L 50 mM Tris (pH 9.00)
  2. Filter both 1 L solutions.
  3. Defrost cells from 09/28/11 in a water bath.
  4. Sonicate cells: 30 s on, 30 s off 3 times.
  5. Centrifuge at 17000 rpm for 1.5 hr.
  6. Run dialysis overnight on supernatant in the 2 batches of 4L of 50 mM Tris (pH 9).

Data

Notes



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