User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Properties of AuNP</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Objective== | ==Objective== | ||
To try to stop the unfolding process of BSA and to determine in what situations BSA/AuNP fibers are stable. | |||
==Description== | ==Description== | ||
# Make two solutions of AuNP. | |||
#* Solution 1 (aqueous AuNP): | |||
#*# Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl<sub>4</sub> in a test tube. | |||
#*# Place in 80°C oven until solution turns purple. | |||
#* Solution 2 (AuNP/BSA fibers): | |||
#*# Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl<sub>4</sub> in a test tube. | |||
#*# Place in 80°C oven for 2 hr. | |||
# Remove solution 1 from oven. | |||
# Add 100 μL of 5.02 mM tris (pH 10.1) to 1 mL of solution 1. | |||
# Take UV vis data. | |||
# Repeat steps 3 and 4. | |||
==Data== | ==Data== | ||
<center> | |||
[[Image:Absorbance_vs_wavelength_11-15-11.jpg|600px]] | |||
</center> | |||
<center> | |||
[[Image:Absorbance_vs_wavelength_magnified_11-15-11.jpg|600px]] | |||
</center> | |||
==Notes== | ==Notes== | ||
Solution 1 began to turn purple after 1.5 hr in the oven, at which point it was removed. | |||
Small purple fibers formed in solution 2. | |||
Revision as of 13:08, 16 November 2011
Properties of AuNP | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo try to stop the unfolding process of BSA and to determine in what situations BSA/AuNP fibers are stable. Description
Data
NotesSolution 1 began to turn purple after 1.5 hr in the oven, at which point it was removed. Small purple fibers formed in solution 2.
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