User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/15: Difference between revisions

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# Make two solutions of AuNP.
# Make two solutions of AuNP.
#* Solution 1 (aqueous AuNP):
#* Solution 1 (aqueous AuNP):
#*# Combine 0.516 mL of BSA and 0.350 mL of HAuCl<sub>4</sub> in a test tube.
#*# Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl<sub>4</sub> in a test tube.
#*# Place in 80°C oven for 1.5 hr.
#*# Place in 80°C oven until solution turns purple.
#* Solution 2 (AuNP/BSA fibers):
#* Solution 2 (AuNP/BSA fibers):
#*# Combine 0.516 mL of BSA and 0.700 mL of HAuCl<sub>4</sub> in a test tube.
#*# Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl<sub>4</sub> in a test tube.
#*# Place in 80°C oven for 2 hr.
#*# Place in 80°C oven for 2 hr.
# Remove solution 1 from oven.
# Remove solution 1 from oven.
# Add 100 μL of 5.02 mM Tris (pH 10.1) to 1 mL of solution 1.
# Add 100 μL of 5.02 mM tris (pH 10.1) to 1 mL of solution 1.
# Take UV vis data.
# Take UV vis data.
# Repeat steps 3 and 4.
# Repeat steps 3 and 4.
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==Data==
==Data==


<center>
[[Image:Absorbance_vs_wavelength_11-15-11.jpg|600px]]
</center>


<center>
[[Image:Absorbance_vs_wavelength_magnified_11-15-11.jpg|600px]]
</center>


==Notes==
==Notes==


Solution 1 began to turn purple after 1.5 hr in the oven, at which point it was removed.
Small purple fibers formed in solution 2.




Revision as of 13:08, 16 November 2011

Properties of AuNP <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To try to stop the unfolding process of BSA and to determine in what situations BSA/AuNP fibers are stable.

Description

  1. Make two solutions of AuNP.
    • Solution 1 (aqueous AuNP):
      1. Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl4 in a test tube.
      2. Place in 80°C oven until solution turns purple.
    • Solution 2 (AuNP/BSA fibers):
      1. Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl4 in a test tube.
      2. Place in 80°C oven for 2 hr.
  2. Remove solution 1 from oven.
  3. Add 100 μL of 5.02 mM tris (pH 10.1) to 1 mL of solution 1.
  4. Take UV vis data.
  5. Repeat steps 3 and 4.

Data


Notes

Solution 1 began to turn purple after 1.5 hr in the oven, at which point it was removed.

Small purple fibers formed in solution 2.



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