User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/15

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Properties of AuNP <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

To try to stop the unfolding process of BSA and to determine in what situations BSA/AuNP fibers are stable.

Description

  1. Make two solutions of AuNP.
    • Solution 1 (aqueous AuNP):
      1. Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl4 in a test tube.
      2. Place in 80°C oven until solution turns purple.
    • Solution 2 (AuNP/BSA fibers):
      1. Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl4 in a test tube.
      2. Place in 80°C oven for 2 hr.
  2. Remove solution 1 from oven.
  3. Add 100 μL of 5.02 mM tris (pH 10.1) to 1 mL of solution 1.
  4. Take UV vis data.
  5. Repeat steps 3 and 4.

Data


Notes

Solution 1 began to turn purple after 1.5 hr in the oven, at which point it was removed.

Small purple fibers formed in solution 2.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/11/15&oldid=566816"