User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/16: Difference between revisions
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Also, to repeat the experiment done on [User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/11/15|11/15/11] | Also, to repeat the experiment done on [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/11/15|11/15/11]]. | ||
==Description== | ==Description== | ||
# Make two solutions of AuNP. | |||
#* Solution 1 (aqueous AuNP): | |||
#*# Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl<sub>4</sub> in a test tube. | |||
#*# Place in 80°C oven until solution turns purple. | |||
#* Solution 2 (AuNP/BSA fibers): | |||
#*# Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl<sub>4</sub> in a test tube. | |||
#*# Place in 80°C oven for 3.5 hr. | |||
# Remove 1 mL of solution 1, place remainder back in oven. | |||
# Take UV vis data. | |||
# Add 100 μL 50.1 mM tris buffer (pH 10.1). | |||
# Repeat steps 3 and 4. | |||
# After 1.5 hr, remove solution 1 from oven again. | |||
# Take UV vis data. | |||
# Add 100 μL 50.1 mM tris buffer (pH 10.1). | |||
# Repeat steps 7 and 8. | |||
==Data== | |||
<b>Absorbance data taken right after nanoparticle formation:</b> | |||
<center> | |||
[[Image:Absorbance vs wavelength 11-16-11.jpg|600px]] | |||
</center> | |||
<center> | |||
[[Image:Absorbance vs wavelength magnified 11-16-11.jpg|600px]] | |||
</center> | |||
<b>Absorbance data taken 1.5 hr after nanoparticle formation:</b> | |||
<center> | |||
[[Image:Absorbance vs wavelength 11-16-11 2.jpg|600px]] | |||
</center> | |||
<center> | |||
[[Image:Absorbance vs wavelength magnified 11-16-11 2.jpg|600px]] | |||
</center> | |||
==Notes== | ==Notes== | ||
Solution 1 began to turn purple after 1.5 hr. | |||
Purple fibers began to form at around 1 hr. | |||
[[Category:Course]] | [[Category:Course]] |
Revision as of 15:24, 19 November 2011
Properties of AuNP II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo try to stop the unfolding process of BSA and to determine in what situations BSA/AuNP fibers are stable.
Description
DataAbsorbance data taken right after nanoparticle formation:
NotesSolution 1 began to turn purple after 1.5 hr. Purple fibers began to form at around 1 hr.
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