User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/16

Objective

To try to stop the unfolding process of BSA and to determine in what situations BSA/AuNP fibers are stable.

Also, to repeat the experiment done on 11/15/11.

Description

1. Make two solutions of AuNP.
   • Solution 1 (aqueous AuNP):
     1. Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl₄ in a test tube.
     2. Place in 80°C oven until solution turns purple.
   • Solution 2 (AuNP/BSA fibers):
     1. Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl₄ in a test tube.
     2. Place in 80°C oven for 3.5 hr.
2. Remove 1 mL of solution 1, place remainder back in oven.
3. Take UV vis data.
4. Add 100 μL 50.1 mM tris buffer (pH 10.1).
5. Repeat steps 3 and 4.
6. After 1.5 hr, remove solution 1 from oven again.
7. Take UV vis data.
8. Add 100 μL 50.1 mM tris buffer (pH 10.1).
9. Repeat steps 7 and 8.

Data

Absorbance data taken right after nanoparticle formation:

Absorbance data taken 1.5 hr after nanoparticle formation:

Notes

Solution 1 began to turn purple after 1.5 hr.

Purple fibers began to form at around 1 hr.