User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/30: Difference between revisions

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==Description==
==Description==


# Make 4 solutions of AuNP:
#* Solutions 1 and 2 (Au/BSA mole ratio = 70)
#*# Combine 1 mL of 2.9 mM HAuCl<sub>4</sub> and 2.41 mL of 0.0172 mM BSA in a test tube.
#*# Dilute to 9 mL with water.
#*# Place in oven at 70°C.
#* Solutions 3 and 4 (Au/BSA mole ratio = 166)
#*# Combine 1 mL of 2.9 mM HAuCl<sub>4</sub> and 1.02 mL of 0.0172 mM BSA in a test tube.
#*# Dilute to 9 mL with water.
#*# Place in oven at 70°C.
# Leave solutions 1 and 3 in oven for 3.5 hr.
# Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.


==Data==
==Data==




Solution 1: Au/BSA = 70, left in the oven
<center>[[Image:70-in.JPG|300px]]</center>
Solution 2: Au/BSA = 70, taken out of the oven every 30 minutes
<center>[[Image:70-in_and_out.JPG|300px]]</center>
Solution 3: Au/BSA = 166, left in the oven
<center>[[Image:166-in.JPG|300px]]</center>
Solution 4: Au/BSA = 166, taken out of the oven every 30 minutes
<center>[[Image:166-in_and_out.JPG|300px]]</center>


==Notes==
==Notes==


Purple fibers began to form in solutions 3 and 4 after 1 hr. Purple solution began to form in solution 1 after 1.5 hr.




Revision as of 10:07, 6 December 2011

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Objective

To try to control the aggregation process of AuNP/BSA.

Description

  1. Make 4 solutions of AuNP:
    • Solutions 1 and 2 (Au/BSA mole ratio = 70)
      1. Combine 1 mL of 2.9 mM HAuCl4 and 2.41 mL of 0.0172 mM BSA in a test tube.
      2. Dilute to 9 mL with water.
      3. Place in oven at 70°C.
    • Solutions 3 and 4 (Au/BSA mole ratio = 166)
      1. Combine 1 mL of 2.9 mM HAuCl4 and 1.02 mL of 0.0172 mM BSA in a test tube.
      2. Dilute to 9 mL with water.
      3. Place in oven at 70°C.
  2. Leave solutions 1 and 3 in oven for 3.5 hr.
  3. Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.

Data

Solution 1: Au/BSA = 70, left in the oven


Solution 2: Au/BSA = 70, taken out of the oven every 30 minutes


Solution 3: Au/BSA = 166, left in the oven


Solution 4: Au/BSA = 166, taken out of the oven every 30 minutes

Notes

Purple fibers began to form in solutions 3 and 4 after 1 hr. Purple solution began to form in solution 1 after 1.5 hr.



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