User:Elaine Marie Robbins/Notebook/CHEM-496/2011/12/06: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(4 intermediate revisions by the same user not shown)
Line 27: Line 27:
# Leave solutions 1 and 3 in oven for 3.5 hr.
# Leave solutions 1 and 3 in oven for 3.5 hr.
# Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.
# Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.
# Take UV vis data of solutions 1 and 2.
# Centrifuge 1 mL of solutions 3 and 4 for 10 min at 13200 rpm.
# Take UV vis data of the supernatant.


==Data==
==Data==




Au/BSA = 70, left in the oven
Solution 1: Au/BSA = 70, left in the oven


<center>[[Image:70-in.JPG|300px]]</center>
<center>[[Image:70-in.JPG|300px]]</center>
Line 37: Line 40:




Au/BSA = 70, taken out of the oven every 30 minutes
Solution 2: Au/BSA = 70, taken out of the oven every 30 minutes


<center>[[Image:70-out.JPG|300px]]</center>
<center>[[Image:70-out.JPG|300px]]</center>
Line 43: Line 46:




Au/BSA = 160, left in the oven
Solution 3: Au/BSA = 166, left in the oven


<center>[[Image:160-in.JPG|300px]]</center>
<center>[[Image:160-in.JPG|300px]]</center>
Line 49: Line 52:




Au/BSA = 160, taken out of the oven every 30 minutes
Solution 4: Au/BSA = 166, taken out of the oven every 30 minutes


<center>[[Image:160-out.JPG|300px]]</center>
<center>[[Image:160-out.JPG|300px]]</center>
<center>[[Image:Absorbance vs wavelength 12-06-11.jpg|600px]]</center>
<center>[[Image:Absorbance vs wavelength magnified 12-06-11.jpg|600px]]</center>


==Notes==
==Notes==

Revision as of 12:23, 7 December 2011

Properties of AuNP IV <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

To try to control the aggregation process of AuNP/BSA.


In addition, to repeat the experiment performed on 11/30/11.

Description

  1. Make 4 solutions of AuNP:
    • Solutions 1 and 2 (Au/BSA mole ratio = 70)
      1. Combine 0.5 mL of 2.9 mM HAuCl4 and 1.20 mL of 0.0172 mM BSA in a test tube.
      2. Add 5 mL of water.
      3. Place in oven at 70°C.
    • Solutions 3 and 4 (Au/BSA mole ratio = 166)
      1. Combine 0.5 mL of 2.9 mM HAuCl4 and 0.51 mL of 0.0172 mM BSA in a test tube.
      2. Add 5 mL of water.
      3. Place in oven at 70°C.
  2. Leave solutions 1 and 3 in oven for 3.5 hr.
  3. Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.
  4. Take UV vis data of solutions 1 and 2.
  5. Centrifuge 1 mL of solutions 3 and 4 for 10 min at 13200 rpm.
  6. Take UV vis data of the supernatant.

Data

Solution 1: Au/BSA = 70, left in the oven


Solution 2: Au/BSA = 70, taken out of the oven every 30 minutes


Solution 3: Au/BSA = 166, left in the oven


Solution 4: Au/BSA = 166, taken out of the oven every 30 minutes




Notes

Results are similar to those observed on 11/30/2011.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/12/06&oldid=571335"