User:Elaine Marie Robbins/Notebook/CHEM-496/2011/12/07: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Properties of AuNP V</span>
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==Objective==
To try to control the aggregation process of AuNP/BSA.
==Description==
# Make 4 solutions of AuNP:
#* Solutions 1 and 2 (Au/BSA mole ratio = 35)
#*# Combine 0.5 mL of 2.5 mM HAuCl<sub>4</sub> and 0.0706 mL of 0.0177 mM BSA in a test tube.
#*# Add 5 mL of water.
#*# Place in oven at 70°C.
#* Solutions 3 and 4 (Au/BSA mole ratio = 100)
#*# Combine 0.5 mL of 2.5 mM HAuCl<sub>4</sub> and 2.02 mL of 0.0177 mM BSA in a test tube.
#*# Add 5 mL of water.
#*# Place in oven at 70°C.
# Leave solutions 1 and 3 in oven for 3.5 hr.
# Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.
==Data==
Solution 1: Au/BSA = 35, left in the oven
<center>[[Image:Photo3A.JPG|300px]]</center>
Solution 2: Au/BSA = 35, taken out of the oven every 30 minutes
<center>[[Image:Photo2A.JPG|300px]]</center>




Solution 3: Au/BSA = 100, left in the oven


<center>[[Image:Photo4A.JPG|300px]]</center>


==Objective==
*Add your objective here
stuff [[User:Matt Hartings|Matt Hartings]] 21:41, 6 September 2011 (EDT)


==Description==
Solution 4: Au/BSA = 100, taken out of the oven every 30 minutes
*Add description here
more stuff [[User:Matt Hartings|Matt Hartings]] 21:43, 6 September 2011 (EDT)


==Data==
<center>[[Image:Photo1A.JPG|300px]]</center>
* Add data and results here...


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.


Solution 1 began to turn purple after 2 hr.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
[[Category:Course]]
[[Category:Miscellaneous]]
[[Category:Miscellaneous]]





Revision as of 13:49, 7 December 2011

Properties of AuNP V <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To try to control the aggregation process of AuNP/BSA.

Description

  1. Make 4 solutions of AuNP:
    • Solutions 1 and 2 (Au/BSA mole ratio = 35)
      1. Combine 0.5 mL of 2.5 mM HAuCl4 and 0.0706 mL of 0.0177 mM BSA in a test tube.
      2. Add 5 mL of water.
      3. Place in oven at 70°C.
    • Solutions 3 and 4 (Au/BSA mole ratio = 100)
      1. Combine 0.5 mL of 2.5 mM HAuCl4 and 2.02 mL of 0.0177 mM BSA in a test tube.
      2. Add 5 mL of water.
      3. Place in oven at 70°C.
  2. Leave solutions 1 and 3 in oven for 3.5 hr.
  3. Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.

Data

Solution 1: Au/BSA = 35, left in the oven


Solution 2: Au/BSA = 35, taken out of the oven every 30 minutes


Solution 3: Au/BSA = 100, left in the oven


Solution 4: Au/BSA = 100, taken out of the oven every 30 minutes

Notes

Solution 1 began to turn purple after 2 hr.