User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/07: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2012/02/07 Entry for User:Elaine_Marie_Robbins/Notebook/CHEM-496)
 
(5 intermediate revisions by the same user not shown)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #cdde95;" align="center"|
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Effects of pH on AuNP</span>
<!-- ## START calendar column -->
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
<LNCalendar></LNCalendar>
|-
<!-- ## END calendar column -->
| colspan="2"|
|align="center" style="background-color: #e5edc8;" |
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ## START search column: Place your logo here. Try keep it below 200px in width and 150px in height. ##  -->
[[Image:BDLlogo_300.png|300px]] <sitesearch>title=Search this Project</sitesearch>
<!-- ## END search column  ## -->
|-
|colspan="2" style="background-color: #F2F2F2;" align="right"|[[{{FULLPAGENAME}}/Entry_Base|Customize your entry pages]] [[Help:Notebook/Project_Base/Customize_entry_page|<html><img src="/images/a/aa/Help.png" border="0" /></html>]]
|-  
|colspan="2"|


==Objective==


To determine the effect of Tris buffers of different pH on a AuNP solution.


==Description==
# Take UV-vis data of 70 Au/BSA ratio solution prepared on [[User:Elaine_Marie_Robbins/Notebook/CHEM-496/2012/01/31|01/31/12]].
# Take UV-vis data of 0.9 mL of each AuNP solution + 0.1 mL of the following pH values of 50 mM Tris:
#* pH 7.5
#* pH 10.1
#* pH 11.85
==Data==


*Graph of absorbance vs wavelength:
<center>[[Image:Absorbance vs wavelength 2-7-12.jpg]]</center>


==Objective==
*Add your objective here
stuff [[User:Matt Hartings|Matt Hartings]] 21:41, 6 September 2011 (EDT)


==Description==
*Add description here
more stuff [[User:Matt Hartings|Matt Hartings]] 21:43, 6 September 2011 (EDT)


==Data==
There appears to be a small shift toward UV and slightly increased absorbance with high pH values.
* Add data and results here...


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.


 
The pH meter used to determine the pH values of 50 mM tris solutions was having difficulty being calibrated.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
[[Category:Course]]
[[Category:Miscellaneous]]
[[Category:Miscellaneous]]




Revision as of 12:44, 7 February 2012

Effects of pH on AuNP <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

To determine the effect of Tris buffers of different pH on a AuNP solution.

Description

  1. Take UV-vis data of 70 Au/BSA ratio solution prepared on 01/31/12.
  2. Take UV-vis data of 0.9 mL of each AuNP solution + 0.1 mL of the following pH values of 50 mM Tris:
    • pH 7.5
    • pH 10.1
    • pH 11.85

Data

  • Graph of absorbance vs wavelength:


There appears to be a small shift toward UV and slightly increased absorbance with high pH values.

Notes

The pH meter used to determine the pH values of 50 mM tris solutions was having difficulty being calibrated.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Elaine_Marie_Robbins/Notebook/CHEM-496/2012/02/07&oldid=581695"