User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/07: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 23: Line 23:
*Graph of absorbance vs wavelength:
*Graph of absorbance vs wavelength:
<center>[[Image:Absorbance vs wavelength 2-7-12.jpg]]</center>
<center>[[Image:Absorbance vs wavelength 2-7-12.jpg]]</center>
There appears to be a small shift toward UV and slightly increased absorbance with high pH values.


==Notes==
==Notes==

Revision as of 12:44, 7 February 2012

Effects of pH on AuNP <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

To determine the effect of Tris buffers of different pH on a AuNP solution.

Description

  1. Take UV-vis data of 70 Au/BSA ratio solution prepared on 01/31/12.
  2. Take UV-vis data of 0.9 mL of each AuNP solution + 0.1 mL of the following pH values of 50 mM Tris:
    • pH 7.5
    • pH 10.1
    • pH 11.85

Data

  • Graph of absorbance vs wavelength:


There appears to be a small shift toward UV and slightly increased absorbance with high pH values.

Notes

The pH meter used to determine the pH values of 50 mM tris solutions was having difficulty being calibrated.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Elaine_Marie_Robbins/Notebook/CHEM-496/2012/02/07&oldid=581695"