User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/14: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Absorbance vs Tris Concentration</span>
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==Objective==
To continue the experiment performed on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/01| 02/01/12]] to determine the effects of various concentrations of tris buffer on AuNP absorbance.
==Description==
# Take UV-vis data for various dilutions of tris buffer (pH 10.9)
#* 1 M
#* 500 mM
#* 200 mM
#* 100 mM
#* 75 mM
==Data==
* Graph of absorbance vs wavelength for AuNP and different dilutions of tris
<center>[[Image:Absorbance vs wavelength magnified.jpg]]</center>
While all concentrations of tris buffer cause a peak shift towards shorter wavelengths, the higher concentrations tended to yield lower absorbance values.




* Graph of peak absorbance vs -log(ionic strength of tris buffer solution).


<center>[[Image:Peak absorbance vs -log(ionic strength) 2-14-12.jpg]]</center>


==Objective==
*Add your objective here
stuff [[User:Matt Hartings|Matt Hartings]] 21:41, 6 September 2011 (EDT)


==Description==
* Graph of peak wavelength vs -log(ionic strength of tris buffer solution).
*Add description here
more stuff [[User:Matt Hartings|Matt Hartings]] 21:43, 6 September 2011 (EDT)


==Data==
<center>[[Image:Peak wavelength vs -log(ionic strength) 2-14-12.jpg]]</center>
* Add data and results here...


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.




Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
[[Category:Course]]
[[Category:Miscellaneous]]
[[Category:Miscellaneous]]




Revision as of 12:50, 21 February 2012

Absorbance vs Tris Concentration <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To continue the experiment performed on 02/01/12 to determine the effects of various concentrations of tris buffer on AuNP absorbance.

Description

  1. Take UV-vis data for various dilutions of tris buffer (pH 10.9)
    • 1 M
    • 500 mM
    • 200 mM
    • 100 mM
    • 75 mM

Data

  • Graph of absorbance vs wavelength for AuNP and different dilutions of tris

While all concentrations of tris buffer cause a peak shift towards shorter wavelengths, the higher concentrations tended to yield lower absorbance values.


  • Graph of peak absorbance vs -log(ionic strength of tris buffer solution).


  • Graph of peak wavelength vs -log(ionic strength of tris buffer solution).

Notes



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